LOCUS       40924_49157        4487 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD029, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..428
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons or Escherichia coli origins to be 
                     assembled with a cyanobacterial replicon; synthetic"
     misc_feature    429..434
                     /label=MfeI
                     /note="MfeI"
     misc_feature    434..439
                     /label=SacI
                     /note="SacI"
     terminator      445..539
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(741..752)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     terminator      complement(2083..2169)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2176..2181
                     /label=NheI
                     /note="NheI"
     misc_feature    2182..2202
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    2200..2205
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2224..2229
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(2266..2282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2290..2306)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2314..2344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2359..2380)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2668..3256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3430..4287)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4288..4392)
                     /label=AmpR promoter
     misc_feature    4418..4423
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc cccgggcccg
      421 ggcccgatca attgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac
      481 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
      541 caagcactag gcgatttttt attaaaacgt ctcaaaatcg tttctgagac gttttagcgt
      601 ttatttcgtt tagttatcgg cataatcgtt aaaacaggcg ttatcgtagc gtaaaagccc
      661 ttgagcgtag cgtggctttg cagcgaagat gttgtctgtt agattatgaa agccgatgac
      721 tgaatgaaat aataagcgca gcgcccttct atttcggttg gaggaggctc aagggagtat
      781 gagggaatga aattccctca tgggtttgat tttaaaaatt gcttgcaatt ttgccgagcg
      841 gtagcgctgg aaaatttttg aaaaaaattt ggaatttgga aaaaaatggg gggaaaggaa
      901 gcgaattttg cttccgtact acgacccccc attaagtgcc gagtgccaat ttttgtgcca
      961 aaaacgctct atcccaactg gctcaagggt ttaaggggtt tttcaatcgc caacgaatcg
     1021 ccaacgtttt cgccaacgtt ttttataaat ctatatttaa gtagctttat tgttgttttt
     1081 atgattacaa agtgatacac taactttata aaattatttg attggagttt tttaaatggt
     1141 gatttcagaa tcgaaaaaaa gagttatgat ttctctgaca aaagagcaag ataaaaaatt
     1201 aacagatatg gcgaaacaaa aaggtttttc aaaatctgcg gttgcggcgt tagctataga
     1261 agaatatgca agaaaggaat cagaacaaaa aaaataagcg aaagctcgcg tttttagaag
     1321 gatacgagtt ttcgctactt gtttttgata aggtaattat atcatggcta ttaaaaatac
     1381 taaagctaga aattttggat ttttattata tcctgactca attcctaatg attggaagga
     1441 aaaattagag agtttgggcg tatctatggc tgtcagtcct ttacacgata tggacgaaaa
     1501 aaaagataaa gatacatgga atagtagtga tgttatacga aatggaaagc actataaaaa
     1561 accacactat cacgttatat atattgcacg aaatcctgta acaatagaaa gcgttaggaa
     1621 caagattaag cgaaaattgg ggaatagttc agttgctcat gttgagatac ttgattatat
     1681 caaaggttca tatgaatatt tgactcatga atcaaaggac gctattgcta agaataaaca
     1741 tatatacgac aaaaaagata ttttgaacat taatgatttt gatattgacc gctatataac
     1801 acttgatgaa agccaaaaaa gagaattgaa gaatttactc ttagatatag tggatgacta
     1861 taatttggta aatacaaaag atttaatggc ttttattcgc cttaggggag cggagtttgg
     1921 aattttaaat acgaatgatg taaaagatat tgtttcaaca aactctagcg cctttagatt
     1981 atggtttgag ggcaattatc agtgtggata tagagcaagt tatgcaaagg ttcttgatgc
     2041 tgaaacgggg gaaataaaat gacactaggt ctagggcggc ggatttgtcc tactcaggag
     2101 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     2161 ttttatttga tgcctgctag catcgcgcgc gcgcgcgcgg atatcctcgg tacccgggga
     2221 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     2281 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2341 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2401 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2461 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2521 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2581 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2641 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2701 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2761 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2821 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2881 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2941 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3001 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3061 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3121 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3181 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3241 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3301 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3361 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3421 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3481 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3541 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3601 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3661 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3721 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3781 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3841 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3901 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3961 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4021 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4081 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4141 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4201 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4261 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4321 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4381 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     4441 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
